Suppressing the Migration of Human Breast Cancer Cell Line by Targeting VAMP3 with miR-199a-3p

  • Nouf N. Laqtom King Abdulaziz University
  • Khloud M. Algothmi King Abdulaziz University
Keywords: microRNA; Breast cancer; Metastasis; miR-199a-3p; Vesicle associated membrane protein

Abstract

Deregulation of microRNAs contributes to multiple processes in cancer growth and progression. miR-199a-3p is decreased in highly metastatic breast cancer cells, MDA-MB-231, and its ectopic expression has a potent antimetastatic effect on these cells. However, the mechanism by which miR-199a-3p mediates its antimetastatic function has yet to be elucidated. Because miR-199a-3p reduces the expression levels of its target genes, it is likely to observe an inverse association between miR-199a-3p and its prometastatic target genes at the expression level. The current work determines that the Vesicleassociated membrane protein 3 (VAMP3) expression is increased in highly metastatic breast cancer cells compared to less metastatic cells, Michigan Cancer Foundation-7. The ectopic expression of miR-199a-3p strongly inhibits VAMP3 Messenger RNA and protein in vitro. Herein, it is confirmed that two sites within the 3'-untranslated sequence of VAMP3 Messenger RNA are actively targeted by miR-199a- 3p, discovering a new regulatory mechanism for VAMP3 expression. Functional studies reveal that the suppression of VAMP3 contributes to miR-199a-3p antimetastatic effect, particularly cellular migration in vitro. In conclusion, these results indicate that miR-199a-3p targeting of VAMP3 possesses a significant potential impact in preventing or curing metastatic breast cancers.

Author Biographies

Nouf N. Laqtom, King Abdulaziz University

Department of Biological Sciences, Division of Genomics and Biotechnology

Khloud M. Algothmi, King Abdulaziz University

Department of Biological Sciences, Division of Genomics and Biotechnology

Published
2016-06-30
How to Cite
Laqtom, N. N., & Algothmi, K. M. (2016). Suppressing the Migration of Human Breast Cancer Cell Line by Targeting VAMP3 with miR-199a-3p. Journal of King Abdulaziz University - Medical Sciences, 23(2), 29-37. https://doi.org/10.4197/Med.23-2.4
Section
Articles